Estimation of Median Lethal Concentration (LC50) Value and Its Confidence Interval for the Effect of Carbamate Pesticide (Methiocarb) on Caenorhabditis Elegans

EasyChair Preprint no. 8937

Abstract

Caenorhabditis elegans increasingly is attractive as a toxicity test organism, particularly as a model system to study mechanisms of toxicity at a molecular level and the way that these lead to whole organism and population level effects. In this study, lethal concentration (LC50) values of methiocarb on nematodes (Caenorhabditis elegans) were investigated. In practice, experimental setup was constituted 30 worms (a total of 300 worms with 30 control worms) to be placed in three replicates. methiocarb was added into NGM at the concentration range from 1-20 mg/l (1, 2.5, 5, 7.5, 10, 12.5, 15, 17.5 and 20 mg/l) and determined mortality of worms exposed to calculated Percentage death of worms in these concentrations, mortality was observed at all treatments. The results indicate that due to methiocarb, can be lethargy, lack of breath in media at all of the concentrations, the reason of death. The results of regression analysis indicated that the mortality rate (Y) is positively correlated the concentration (X) having a regression coefficient (R), after 24 hours LC50 value (with 95% confidence limits) was estimated at 4.805 mg/l.

Keyphrases: Caenorhabditis elegans., Median Lethal Concentration (LC50), MS Excel software 2019

@Booklet{EasyChair:8937,
  author = {Krishna Rana and Priyanka Gautam},
  title = {Estimation of Median Lethal Concentration (LC50) Value and Its Confidence Interval for the Effect of Carbamate Pesticide (Methiocarb) on Caenorhabditis Elegans},
  howpublished = {EasyChair Preprint no. 8937},

  year = {EasyChair, 2022}}